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PROSPERO: PRediction of Outcome using Sequence & Protein Experimental Results Online 1.0

DLS | Sequence Analysis | SEC | TM | Yield

DLS Data

Dynamic Light Scattering

Desired inputs are the following inputs for each peak found. There can be up to 3 peaks. The most significant peak must be selected as the major peak.

Variable	Description	Range ^{Possible Values}	Units	Calculation
DLS_P	Percent polydispersity.	[0 - 100]	%	From histogram, for major peak.
DLS_I	Percent intensity	[0 - 100]	%	For major peak, P_m among n peaks & m ≤ n : P_m / ( P_m + P_{m + 1} ... P_n )
DLS_MW	Molecular Weight as calculated from the R_H	>0	kilodalton (kDa)
DLS_MR	Molecular Ratio	>0		of DLS_MW / (calculated molecular weight of monomer)
R_H	Hydrodynamic radius.	> 0	nanometer (nm)

DLS Data Import

The site is configured to accept certain machine outputs to alleviate data entry tasks. One example is the DYNAMICS specific file format. The following is a sample of the DYNAMICS experiment ouput in image and text form.


Results Summary:

		D (E-9cm^2/s)	R (nm)	MW (kDa)	Polyd (nm)	%Polyd	Polyd Index
Cumu Monomodal		179.9	     5.755	     203.5	     4.674	81.1	0.67

Cumu Bimodal 1		300.0	     3.492	     64.30						    
Cumu Bimodal 2		64.07	     17.42	     3037.						    

Regu I Peak  1		6511.	    0.1583	   0.04512	    0.0000	 0.0	0.00
Regu I Peak  2		290.5	     3.859	     79.31	     1.143	29.6	0.09
Regu I Peak  3		31.63	     38.12	1.685e+004	     15.64	41.0	0.17

Regu M Peak  1		6511.	    0.1583	   0.04512	    0.0000	 0.0	0.00
Regu M Peak  2		356.8	     3.065	     46.29	    0.8176	26.7	0.07
Regu M Peak  3		43.96	     25.45	     6547.	     8.670	34.1	0.12

------------------------	
Regularization Results:	
Delay Time (s)	D (E-9cm^2/s)	    R (nm)	   %Intensity	   %Mass
7.31e-006	     6511.	    0.1583	     2.07	     99.7
9.69e-006	     4914.	    0.2098	    0.000	    0.000
9.20e-005	     517.2	     1.993	     1.48	   0.0356
 0.000122	     390.3	     2.641	     17.2	    0.178
 0.000162	     294.6	     3.500	     21.9	   0.0974
 0.000214	     222.3	     4.637	     16.9	   0.0324
 0.000284	     167.8	     6.145	     8.01	  0.00659
 0.000376	     126.6	     8.142	    0.000	    0.000
 0.000874	     54.43	     18.94	     4.19	 0.000118
  0.00116	     41.08	     25.10	     6.94	8.38e-005
  0.00154	     31.00	     33.25	     7.88	4.09e-005
  0.00203	     23.40	     44.06	     6.92	1.54e-005
  0.00270	     17.66	     58.38	     4.66	4.47e-006
  0.00357	     13.33	     77.36	     1.87	7.73e-007
  0.00473	     10.06	     102.5	    0.000	    0.000