|BIOC 426: Basic Techniques in Biochemistry (lab course)|
I. Protein isolation (a) Basis of affinity chromatography (b) Dissociation constants, relative affinities (c) Isozymes (d) Native gel electrophoresis (e) SDS-PAGE analysis of purity (f) Quantitative protein determination (g) Enzymatic reactions, specific activity, reaction mechanisms (h) Kinetic analysis of two-substrate reactions II. Immunological techniques (a) The antibody response, B cells, T cells, antigen presentation (b) Structure and function of IgG (c) Isolation of IgY from egg yolk (d) Monoclonal and polyclonal antibodies (e) Salt precipitation of proteins (f) Biotinylation of proteins (g) Streptavidin-biotin interaction (h) Principles of ELISA (i) Principles of Western blot III. Recombinant DNA techniques (a) Gene structure, exons, introns, mutations (b) DsRed fluorescent protein (c) Polymerase chain reaction, amplification of DNA (d) DNA sequencing based on fluorescent dye terminator (e) Sequence analysis with BLAST through NCBI website (f) Preparation of a cloning vector (g) Cloning of PCR fragment (h) Transformation of E. coli, blue/white colony selection (i) Mini-prep of plasmid DNA (j) Restriction enzyme digestion of amplimers and plasmid DNA (k) Agarose gel electrophoresis
Students will be graded on lab participation (10%), 3 quizes (40%), and 3 written reports (50%).
Grade history: median 3.2 out of 4.0
|CONJ 545: Molecular interactions and Medicine|
Note that this is NOT a training course in using a particular molecular software package.
The course will assume knowledge at the level of an advanced undergraduate level of biochemistry,
math and physics.
Be aware that principles of physical chemistry and equations will not be eschewed.
Students will be graded on in-class participation and a final written exam. Participation includes in-class
assignments and the demonstration of familiarity with assigned reading during in-class discussions.
Grade history: median 3.5 out of 4.0
Andrew R. Leach. "Molecular Modelling: Principles and Applications". Prentice Hall, 2nd edition, 2001.